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The Non-Radioactive Phosphatase Assay Systems provide a fast, convenient and flexible alternative for measuring protein phosphatase activity. These systems determine the amount of free phosphate generated in a reaction by measuring the absorbance of a molybdate:malachite green:phosphate complex. These systems allow the use of a variety of buffer conditions and substrates, including naturally phosphorylated proteins or synthetic phosphopeptides. The effective range for the detection of phosphate released during an assay is 100-4,000pmol of phosphate. In addition to measuring phosphatase activity in partially fractionated and purified samples, the Phosphatase Assay Systems can also measure phosphatase activity in crude cell or tissue extracts. A unique Molybdate Dye Additive that is combined with the Molybdate Dye Solution aids in the solubilization of proteins exposed to the acid conditions of the Molybdate Dye Solution, which alone could potentially cause precipitation of the proteins.
