Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human RSK4 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RSK4 is a member of the 90-kDa ribosomal S6 kinase family which are important components of growth factor mediated stimulation of cellular proliferation, survival and differentiation. RSK4 is activated via coordinated phosphorylation by ERK and 3-phosphoinositide-dependent protein kinase-1 (PDK1). RSK4 is often associated with contiguous gene syndromes consisting of X-linked deafness type 3 (DFN3), mental retardation (MRX) and choroideremia (CHM). RSK4 is most abundantly expressed in the brain and kidney. Kinase Enzyme System contains: RSK4 Kinase, 10ug (Human, recombinant, full-length); MW: ~112kDa. RSK Substrate (1mg/ml; KRRRLSSLRA); derived from human 40S ribosomal protein S6 (amino acids 230-239). 5X Reaction Buffer; 0.1M DTT.
