Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human RIPK2 (1-299) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. RIPK2 (RIP2; RICK) is a death domain-containing protein kinase encoding a predicted 540-amino acid protein that contains an N-terminal serine/threonine kinase catalytic domain and a C-terminal caspase activation and recruitment domain. RIPK2 is thought to regulate apoptosis induced by the FAS receptor pathway (1). RIPK2 has been shown to specifically interact with the CARD of ICE/caspase-1, and this interaction correlates with the processing of pro-caspase-1 and the formation of the active caspase-1 p20 (2). Kinase Enzyme System contains: Kinase: RIPK2, 10ug (Human, recombinant; amino acids 1-299). MW: ~59kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. RIPK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/8767/.
