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The RealTime-Glo Apoptosis and Necrosis Assay is a simple, non-lytic assay allows real-time monitoring of apoptosis progression, without the need for multiple plates, complicated processing, or specialized detection equipment. A multimode reader capable of detecting luminescence and fluorescence is the only instrument required. The assay measures exposure of phosphatidylserine (PS) on the outer leaflet of the cell membrane during the apoptotic process and secondary necrosis. Annexin V binding is detected with a simple luminescence signal, and necrosis is detected with a fluorescence signal.
