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Ramos (HiBiT) TCK Bioassay, Propagation Model
PROM2472
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Cytotoxic mAbs and/or effector cells are incubated with target cells expressing a HiBiT fusion protein. Upon killing of the target cell, the HiBiT fusion protein is released and binds extracellular LgBiT to create a functional NanoBiT Luciferase enzyme. Luminescence is measured using a luciferase substrate and the GloMax Discover System.