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Raji (LDH-HiBiT) CD19-KO TCK Bioassay, CPM
PROM2042
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Cytotoxic mAbs and/or effector cells are incubated with target cells expressing a HiBiT fusion protein. Upon killing of the target cell, the HiBiT fusion protein is released and binds extracellular LgBiT to create a functional NanoBiT Luciferase enzyme. Luminescence is measured using a luciferase substrate and the GloMax Discover System.

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