SSI 8-strips of low-profile PCR tubes, clear or white
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
PowerQuant System is a 5-color, 4-target probe-based qPCR assay that simultaneously quantifies the total amount of amplifiable autosomal and Y-chromosomal DNA in a single assay using the same DNA standards. During amplification, annealing of the probe to its target sequence generates a substrate that is cleaved by the 5' nuclease activity of Taq polymerase when the enzyme extends from an upstream primer into the region of the probe, liberating the fluorescent dye from its proximal position to the quencher. The resulting increase in amplification cycles leads to an increase in fluorescence. The kit contains an internal PCR control to test for false-negative results that may occur in the presence of PCR inhibitors. Advantages: more consistent Auto/ Y ratio; assessment of DNA degradation; reliable sample quality assessment; and flexible options for 4-point to 7-point standard curve. Optimized for use on the AB 7500 real-time PCR systems with v2.0.6 or HID1.1 or higher software versions.
