Bioneer DNA/RNA Preparation Kits
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The PolyATtract mRNA Isolation Systems use MagneSphere technology to isolate mRNA rapidly and effectively from total RNA. They use a biotinylated oligo(dT) primer to hybridize, at high efficiency in solution, to the 3' poly(A)+ region present in most mature eukaryotic mRNAs. The hybrids are bound to streptavidin coupled to paramagnetic particles, captured using a magnetic separation stand and washed at high stringency. mRNA is eluted from the solid phase by adding ribonuclease-free, deionized water. With total RNA as the starting material, poly(A)+ mRNA is isolated in approximately 45 minutes. Isolated mRNA is suitable for molecular biology applications such as in vitro translation and cDNA synthesis. Cat.# Z5200 and Z5210 contain sufficient reagents for 3 separate mRNA isolations, each from 1-5mg of total RNA. Cat.# Z5300 and Z5310 contain sufficient reagents for 15 separate mRNA isolations, each from 100-1,000ug of total RNA. Z5200 and Z5300 also contain a Magnetic Separation Stand.
