Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
NanoLuc Stability Sensors are ready-to-use vector systems that enable stability studies of two key signaling proteins, HIF1A and NRF2, providing a method to directly measure this primary signaling event. The HIF1A Vector System enables simple quantification of intracellular HIF1A protein levels to study the dynamics of this signaling protein in mediating cellular response to hypoxia. It contains a vector encoding NanoLuc fused to the C-terminus of the HIF1A protein under control of the CMV promoter. The NRF2 Vector System enables simple quantification of intracellular NRF2 protein levels to study the dynamics of this signaling protein in mediating cellular response to oxidative stress. It contains a vector encoding NanoLuc fused to the C-terminus of the NRF2 protein under the control of the CMV promoter, a pKEAP1-expressing vector for proper regulation of intracellular NRF2 levels. Both systems include Transfection Carrier DNA for titratable intracellular fusion protein expression.
