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The pNL2.1[Nluc/Hygro] and pNL2.2[NlucP/Hygro] Vectors are used to clone a putative promoter to drive expression of the bright NanoLuc reporter protein. Select for stable expression in mammalian cells using hygromycin. NanoLuc (Nluc) luciferase is a small enzyme (19.1kDa) engineered for optimal performance as a luminescent reporter. The enzyme is about 100-fold brighter than either firefly (Photinus pyralis) or Renilla reniformis luciferase using a novel substrate, furimazine, to produce high intensity, glow-type luminescence. The luminescent reaction is ATP-independent and designed to suppress background luminescence for maximal assay sensitivity.
