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Recombinant full-length human PKAcbeta was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. The catalytic subunit C-beta of PKA (PKAcbeta) is a member of the Ser/Thr protein kinase family (the PKA catalytic subunit consist of three gene products: C-alpha, C-beta, and C-gamma) and has been assigned to human chromosome region 1p36.1. PKAcbeta is derived from a gene distinct from C-alpha and shows tissue-specific expression. At the amino acid level C-alpha and C-beta showed 93% homology. Kinase Enzyme System contains: PKAcbeta Kinase, 10ug (Human, recombinant; full length); MW: ~65kDa. CREBtide Substrate (1mg/ml; KRREILSRRPSYR); derived from human CREB1 isoform A (amino acids 109-121). 5X Reaction Buffer; DTT, 0.1M.
