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Phosphatidylinositol (PI) and its phosphorylated derivates are important second messengers that are critical as signaling molecules and for cellular membrane remodeling. These derivatives are generated by a family of kinases called phosphoinositide lipid kinases (PIKs). Promega lipid kinase enzymes, substrates and detection systems provide a complete set of reagents for performing phosphoinositide lipid kinase (PIK) reactions using a luminescent ADP-detection platform, the ADP-Glo Kinase Assay. The reagents include purified human recombinant proteins of Class I PI3Ks, optimized reaction buffer and ready-to-use lipid kinase substrates. The enzymes are available separately or can be purchased as part of the PI3K-Glo Class I Profiling Kit, which contains PI3Ks (alpha, beta, gamma and delta; 5ug each), PIP2:3PS Lipid Kinase Substrate (0.25mg) and the ADP-Glo Kinase Assay, 1,000 assays.
