Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Creating a cell line with an indicator of a functional signaling pathway is useful for deciphering the components in a signaling pathway. These tools are made by insertion of multiple repeats of a response element upstream of a minimal promoter (minP). Promega has designed vectors that report the activity of a variety of pathways using the optimized luc2 firefly luciferase gene in the pGL4 backbone. These vectors also have a hygromycin resistance selectable marker, allowing use either in transient transfection experiments or for selection of a stable cell line. In addition, some of these predesigned response element vectors also are available stable cell lines (GloResponse Cell Lines) to speed your research.
