Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The NanoLuc protein fusion vectors enable simple generation of N- or C-terminal fusions of NanoLuc luciferase with your protein of interest and are available. The pF Vector series generates N- or C-terminal Nluc fusion proteins using the Flexi Vector Cloning System?a directional cloning method based on two rare-cutting restriction enzymes, SgfI and PmeI, that provides a rapid, efficient and high-fidelity way to transfer protein-coding regions between a variety of Flexi Vectors without the need to resequence. Select the pFN31A and pFN31K Vectors for N-terminal protein fusions and pFC32A and pFC32K Vectors for C-terminal protein fusions. Also make NanoLuc protein fusions with the pNLF Vector series: Generate N- or C-terminal fusions to the full-length Nluc protein or attach secreted Nluc to the N-terminus of the protein of interest using traditional cloning with a multiple cloning site (MCS).
