Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The following vectors are used for inducible expression of HaloTag fusion proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Expression levels depend highly on the nature of the protein, but in general the N-terminal HaloTag fusion protein can increase expression level, enhance refolding and boost solubility of the expressed protein. HaloTag vectors are supplied as Flexi System vectors. Flexi vectors contain a His6-HaloTag dual tag. The dual tag enables protein purification with the reusable Ni-resin while retaining the HaloTag covalent labeling properties. pFN29A/K His6HaloTag T7 Flexi Vectors are designed for protein expression with an N-terminal His6-HaloTag dual tag in E. coli T7 cell-free expression systems. pFC30A/K His6HaloTag T7 Flexi Vectors are designed for protein expression with a C-terminal His6-HaloTag dual tag in E. coli T7 cell-free expression systems.
