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The P450-Glo CYP3A4 Assay provides a homogeneous, luminescent method for measuring cytochrome P450 CYP3A4 activity. The assay employs luminogenic P450 substrates that are derivatives of beetle luciferin, a substrate for luciferase enzymes. The substrates are readily taken up by cells and rapidly converted into luciferin inside the cell, which reduces the incubation time required (typically 30-60 minutes).The assay exhibits exquisite sensitivity, low background signals and broad dynamic range, generating a \"glow-type\" luminescent signal produced using Ultra-Glo Luciferase coupled with a proprietary buffer system. The half-life of the luminescent output is greater than two hours, eliminating the need for luminometers with injectors and allowing batch plate processing.This product is also available as a Screening System.
