Bioneer ExiProgen EC1 Protein Synthesis Kit (automated)
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Recombinant full-length human NEK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. NEK2 is closely related in its catalytic domain to the serine/threonine protein kinase NIMA of Aspergillus nidulans that is required for entry into mitosis and may function in parallel to the universal mitotic inducer p34cdc2. Like NIMA, the NEK2 protein is almost undetectable during G1 but accumulated progressively throughout S, reaching maximal levels in late G2. NEK2 is shown to be expressed most abundantly in the testis of the adult tissues examined being localized to the nucleus. Kinase Enzyme System contains: Kinase: NEK2, 10ug (Human, recombinant full-length). MW: ~76kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. NEK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/4751/.
