Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Most proteins and their abundance are regulated via the ubiquitin proteasome system, which uses ubiquitin conjugation to signal proteins to be trafficked to the proteasome for degradation. Ubiquitination can vary in levels (mono- and poly-) and be mediated through a variety of amino acid linkages. The NanoBRET Ubiquitination Starter Kit provides the tools to create target-specific live-cell ubiquitin assays to globally measure ubiquitination on a target protein. Measure dynamic increases or decreases in relative levels of target protein ubiquitination following cellular treatments, such as small molecules or pathway inducers, that would influence protein stabilization or degradation, respectively. These assays can be particularly useful for studying targeted degradation compounds because the bioluminescent resonance energy transfer ratio of the NanoBRET signal means you can investigate proteasome recruitment while simultaneously monitoring target protein levels to assess degradation.
