CyBio Drop automated liquid handler
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
NanoBRET Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. Based on NanoBRET System, an energy transfer technique designed to measure molecular proximity in living cells, NanoBRET TE BET BRD Assay analyzes apparent affinity of test compounds by competitive displacement of a NanoBRET tracer reversibly bound to a NanoLuc BET BRD fusion protein in cells. Bioluminescence resonance energy transfer is achieved by transferring luminescent energy from NanoLuc luciferase to the fluorescent tracer bound to the target protein-NanoLuc fusion. Compounds applied to cells and specifically engage the intracellular fusion will result in a decrease in BRET. To ensure accurate assessment of intracellular TE, a NanoLuc inhibitor is used to mitigate extracellular NanoLuc signal that may arise from cells compromised during handling, while not adversely affecting NanoLuc luciferase expressed within healthy living cells.
