Harvard Locomotor Activity and Exploration
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Recombinant human MLCK (1425-1776) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. MLCK or myosin light chain kinase is a muscle member of the immunoglobulin gene superfamily and is a calcium/calmodulin-dependent protein kinase. MLCK is a regulatory protein for smooth muscle contraction, which acts by phosphorylating 20kDa myosin light chain (MLC20) to activate the myosin ATPase activity. The kinase activity for the phosphorylation is localized at the central part of MLCK, which is also furnished with actin-binding activity at its N terminal and myosin-binding activity at its C terminal. Kinase Enzyme System contains: Kinase: MLCK, 10microg (Human, recombinant; amino acids 1425-1776). MW: ~70kDa. Substrate: MRCL3 Peptide (KKRPQRATSN-VFAM-NH2); derived from human myosin regulatory light chain MRCL3 (amino acid 11-24). Other: Reaction Buffer, DTT, Ca2+/Calmodulin Solution. MLCK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/91807/.
