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Antibody-dependent cell-mediated cytotoxicity (ADCC) is an important mechanism of action (MOA) of antibodies that target virus-infected or diseased (e.g., tumor) cells for destruction by components of the cell-mediated immune system. Mouse FcgammaRIV (mFcgammaRIV) is the predominant receptor involved in ADCC in the mouse and is more closely related to human FcgammaRIIIa, the primary Fc receptor involved in ADCC in humans, than mFcgammaRIII. The mFcgammaRIV ADCC Reporter Bioassay is a biologically relevant MOA-based assay that can be used to measure the activity of mouse antibodies that specifically bind and activate FcgammaRIV. Mouse IgG2a, and to a lesser extent IgG2b, are known to mediate ADCC through the activation of mFcgammaRIV. In contrast, mouse IgG1 does not bind to mFcgammaIV. The bioassay workflow is simple, robust, compatible with 96-well and 384-well plate formats and provides a quantitative measure of ADCC with low variability and high accuracy.
