Bioneer AccuRapid Cell-Free Protein Expression Kit
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MAO-Glo Assay is a homogeneous luminescent method for measuring monoamine oxidase (MAO) activity from recombinant and native sources and for testing the effects of test compounds on MAO activity. First incubate the MAO enzyme source with the MAO substrate. The MAO Substrate is a derivative of beetle luciferin. Upon reaction with MAO, the derivative is converted into luciferin, which in turn reacts with luciferase to produce light. The amount of light produced is directly proportional to MAO activity. After the reaction, add the reconstituted Luciferin Detection Reagent (LDR). The reagent simultaneously stops the MAO reaction and initiates a stable glow-type luminescent signal with a half-life greater than 5 hours, eliminating the need for strictly timed luminescent detection. The assay includes substrate, two reaction buffers (one that can be used with either MAO A or MAO B enzyme and one that is designed specifically for MAO B), a lyophilized LDR and the Luciferin Detection Buffer.
