Quadra4 automated liquid handling workstation
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
High-throughput, automated purification of sequencing reactions, including BigDye Terminator reactions, is performed using MagneSil GREEN Paramagnetic Particles with standard, nonskirted 96-well amplification plates. No user intervention is required from when plates are placed on the instrument until samples are ready for loading onto the fluorescent DNA sequencer. Purified products are approved for fluorescent sequencing reactions (Phred* 20 quality scores >/= 650 bases). Protocols are available for automated instruments from Beckman Coulter and Tecan; validated automated methods are available at: www.promega.com/automethods/. The Plate Clamp 96 and Plate Stand are recommended for automated use because they ensure PCR plates are uniformly flat for liquid transfer on a robotic instrument. *A Phred score is a widely recognized method to measure DNA sequence quality. Phred is a base-calling program for DNA sequence traces available from Codoncode Corporation.
