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The Lumit FcgammaR Binding Immunoassays are novel homogeneous (no-wash) competition assays to measure the interaction between human Fc receptors and antibodies or Fc fusion proteins. In the assays, a human IgG1 labeled with LgBiT (Tracer-LgBiT) is used as the tracer. A C-terminal biotinylated human FcgammaR bound to Streptavidin-SmBiT (FcgammaR-Biotin-SA-SmBiT) is used as the target. In the absence of an antibody analyte sample, Tracer-LgBiT binds to the FcgammaR-SmBiT target, resulting in maximum luminescence signal. In analyte samples, unlabeled IgG will compete with Tracer-LgBiT for binding to the FcgammaR target, resulting in a concentration-dependent decrease in luminescent signal. These easy-to-use biochemical assays can complement and provide orthogonal data to support results from cell-based functional bioassays.
