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The Kinase-Glo Luminescent Kinase Assays are homogeneous non-radioactive methods to determine purified kinase activity by quantifying the amount of ATP remaining in solution following a kinase reaction. Designed for use with multiwell plate formats, the assays are ideal for automated high-throughput screening and can be used to assay protein, lipid and sugar kinases. The procedure involves adding a single reagent directly to a completed kinase reaction. This results in the generation of a luminescent signal correlated with the amount of ATP present and inversely proportional to the amount of kinase activity. The Kinase-Glo Assays generate a glow-type luminescent signal produced using a patented stabilized luciferase (Ultra-Glo Luciferase) coupled with a proprietary buffer system. The assay produces excellent Z'-factor values of greater than 0.7 in 96- and 384-well formats, easily detects known kinase inhibitors and provides IC50 values comparable to those reported in the literature.
