CyBio Drop automated liquid handler
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Recombinant human ITK (amino acids 352-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ITK is a member of the TEC family of nonreceptor tyrosine kinases. ITK is expressed in T-cells and is important for T-cell development and activation through the antigen receptor. ITK requires prior activation of Lck, Zap-70 and PI3-kinase for efficient activation and shares major substrates with both Lck and Zap-70. ITK knockout mice show multiple effects on T cell development, cytokine production and T-helper cell differentiation. T cells that lack or express mutant versions of ITK show impaired TCR-induced actin polymerization, cell polarization and regulation of the signaling events involved in cytoskeletal reorganization. Kinase Enzyme System contains: Kinase: ITK, 10ug (Human, recombinant; amino acids 352-end). MW: ~53kDa. Substrate: Poly (4:1 Glu, Tyr) Peptide. Other: Reaction Buffer, DTT, MnCl2. ITK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/3702/.
