CyBio Drop automated liquid handler
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The IL-12 Bioassay (Cat.# JA2601, JA2605) is a bioluminescent cell-based assay designed to measure IL-12 stimulation or inhibition. The IL-12 Bioassay Cells are provided in a thaw-and-use format as cryopreserved cells that can be thawed, plated and used in an assay without the need for cell propagation. The IL-12 Bioassay Cells are also available in a Cell Propagation Model (CPM; Cat.# J3042) format, as cryopreserved cells that can be thawed, propagated and banked for long-term use. The IL-12 Bioassay consists of human cells engineered to express the IL-12 receptor and a luciferase reporter driven by a response element (RE). When IL-12 binds to IL-12R, it transduces intracellular signals resulting in luminescence. The bioluminescent signal is detected and quantified using the Bio-Glo Luciferase Assay System (Cat.# G7940, G7941) and a standard luminometer, such as the GloMax Discover System.
