Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human Haspin (GSG2) (amino acids 470-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Haspin is a protein kinase that regulates chromosome and spindle function during mitosis and meiosis. Haspin expression is detected in fetal liver, skin, kidney, small intestine and in all proliferating cells. Haspin phosphorylates H3 thr3 (H3T3ph) in human cell lines and depletion of Haspin by RNA interference reveals that Haspin is required for H3 thr3 phosphorylation in mitotic cells. Kinase Enzyme System contains: Haspin Kinase, 10ug (Human, recombinant; amino acids 470-end). MW: ~64kDa. Histone H3 Peptide Substrate (1mg/ml) (ARTKQTARKS-TGGKAPRKQLAGGKKC); derived from human histone H3 amino acids 1-21. 5X Reaction Buffer, 0.1M DTT.
