Sensor Sense ETD-300 Real-time Ethylene Gas Analyser
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The GSH/GSSG-Glo Assay is a luminescence-based system for detection and quantification of total glutathione (GSH +GSSG), GSSG and GSH/GSSG ratios in cultured cells. A change in GSH levels is an indicator of oxidative stress, potentially leading to apoptosis or cell death. The assay provides a simple, rapid multiwell-plate format where stable luminescent signals are correlated with either the GSH or GSSG concentration of a sample directly in culture wells. Both total glutathione and GSSG determinations are based on the reaction where GSH-dependent conversion of a GSH probe, Luciferin-NT, to luciferin by a glutathione-S-transferase enzyme is coupled to a firefly luciferase reaction. Light from luciferase is dependent on the amount of luciferin formed, which is in turn dependent on the amount of GSH present, making the luminescent signal proportional to the amount of GSH. Determination of total glutathione and GSSG are performed in parallel reactions.
