CyBio Drop automated liquid handler
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The GloSensor cAMP Assay presents a novel approach to measuring cAMP levels in live cells. The assay is based on the GloSensor Technology, a genetically modified form of firefly luciferase into which a cAMP-binding protein moiety has been inserted. Upon binding of cAMP, conformational change is induced, leading to increased light output. Researchers can use the GloSensor cAMP Assay by transiently expressing a receptor of interest and the biosensor in the cell line of choice. Stably transfected cell lines with both the biosensor and the receptor of interest also can be made. The protocol is simple: Cells are pre-equilibrated with GloSensor cAMP Reagent for approximately 2 hours; then cells are treated with specific agonists/antagonists or compounds, and luminescence is measured after 10-30 minutes. Most any common luminometer with injectors is sufficient to read the assay. GloSensor cAMP Reagent is required for use with this assay per the GloSensor Limited Use Label License.
