CyBio Drop automated liquid handler
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human FRK (amino acids 208-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. FRK (fyn-related kinase) or Rak is a nuclear tyrosine kinase and member of the Src sub-family. Restricted expression of FRK is detected in a broad range of cell lines with highest levels in epithelial cells. Increased expression of FRK has been shown in breast and renal cell carcinoma cell lines. In addition the retinoblastoma tumor susceptibility gene product pRb associates with FRK in vitro and in vivo. Overexpression of FRK in beta-cells from the pancreas increases the susceptibility of these cells to beta-cell-toxic events (hallmark of Type I diabetes). Kinase Enzyme System contains: FRK Kinase, 10ug (Human, recombinant; amino acids 208-end). MW: ~60kDa. Poly (Glu4, Tyr1) Peptide Substrate (1mg/ml). 5X Reaction Buffer, 2.5M MnCl2, 0.1M DTT.
