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Recombinant full-length, tag-free human ERK1 was expressed by baculovirus in Sf9 insect cells and activated by active MEK1 in vitro. ERK1 is a protein serine/threonine kinase that is a member of the extracellular signal-regulated kinases (ERKs), which are activated in response to numerous growth factors and cytokines. Activation of ERK1 requires both tyrosine and threonine phosphorylation that is mediated by MEK. ERK1 is ubiquitously distributed in tissues with the highest expression in heart, brain and spinal cord. Activated ERK1 translocates into the nucleus, where it phosphorylates various transcription factors (e.g., Elk-1, c-Myc, c-Jun, c-Fos and C/EBP beta). Kinase Enzyme System contains: Kinase: ERK1, 10ug (Human, recombinant full-length). MW: ~44kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. ERK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5595/.
