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Recombinant full-length human DGKE was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. Diacylglycerol kinase epsilon (DAG kinase epsilon) is the only member of class III diacylglycerol kinase family, which lacks the E-F hand motifs for Ca2+ binding but possess a clear selectivity for arachidonoyl-containing species of diacylglycerol. DGK epsilon might decrease signals conducted via arachidonoyl-diacylglycerol or promotes the synthesis of phospholipids with certain fatty acids (1). DGK epsilon contributes to Huntington disease pathogenesis and its inhibition might be effect in the therapeutics. Kinase Enzyme System contains: DGKE Kinase, 10ug (Human, recombinant, full-length); MW: ~91kDa. Dilauroyl-sn-glycerol Substrate, 10mM. 5X Reaction Buffer; DTT, 0.1M; Lipid Dilution Buffer II.
