Use Carboxy-X-Rhodamine (CXR) Reference Dye (identical to ROX dye) with instruments that require a higher level of reference dye than that in the GoTaq qPCR Master Mix.
Spectrum CE System, 8-Capillary
GoTaq(R) 1-Step RT-qPCR System, 5ml
GoTaq(R) 2-Step RT-qPCR System, 5ml
ProDye(TM) Terminator Sequencing System, 1000rxn
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