Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human c-KIT (544-end) was expressed by baculovirus in Sf9 insect cells using a N-terminal GST tag. c-KIT is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor). c-KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. c-KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in c-KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed curbing neoplastic mast cell growth. Kinase Enzyme System contains: Kinase: c-KIT, 10microg (Human, recombinant; amino acids 544-end). MW: ~73kDa. Substrate: Poly (4:1 Glu, Tyr) peptide. Other: Reaction Buffer, MnCl2, DTT. c-KIT NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/3815/.
