Human Corp Water Purification System
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant full-length human CHK2 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. CHK2 is rapidly phosphorylated and activated in response to replication blocks and DNA damage; the response to DNA damage occurs in an ataxia telangiectasia mutated (ATM)-dependent manner (1). Expression of wild-type CHK2 leads to increased p53 stabilization after DNA damage, whereas expression of a dominant-negative CHK2 mutant abrogated both phosphorylation of p53 on Ser-20 and p53 stabilization (2). Kinase Enzyme System contains: Kinase: CHK2, 10ug (Human, recombinant full-length). MW: ~88kDa. Substrate: Chktide (KKKVSRSGLYRSPSMPENLNRPR); derived from human CDC25C protein isoform A (amino acids 205-225). Other: Reaction Buffer, DTT. CHK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/11200/.
