Bioneer ExiProgen EC1 Protein Synthesis Kit (automated)
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The CheckMate/Flexi Vector Mammalian Two-Hybrid System provides a means to confirm, validate and study suspected interactions between two proteins or domains and can be used to generate stable cell lines for cell-based assays. Developed primarily for mammalian proteins of interest, the system can allow protein expression and post-translational modifications in an environment mimicking the native cell milieu. It is patterned on the yeast two-hybrid system with one protein of interest (X) fused to a DNA-binding domain and the other protein (Y) fused to a transcriptional activation domain. The system relies upon three plasmids that are co-transfected into mammalian cells, each plasmid having unique features. This system differs from the original CheckMate Mammalian Two-Hybrid System in that the vectors are compatible with the Flexi Vector System, which allows directional cloning and rapid, efficient and high-fidelity transfer of protein coding regions between a variety of Flexi Vectors.
