Harvard Locomotor Activity and Exploration
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The Caspase-Glo 1 Inflammasome Assay is a homogeneous, bioluminescent method to selectively measure the activity of caspase-1, a member of the cysteine aspartic acid-specific protease (caspase) family and an essential component of the inflammasome. The assay provides a luminogenic caspase-1 substrate in a lytic reagent optimized for caspase-1 activity and luciferase activity. A single addition of this reagent results in cell lysis, substrate cleavage by caspase-1 and generation of light by the proprietary, thermostable Ultra-Glo Recombinant Luciferase. The coupled-enzyme system reaches a steady-state between caspase cleavage of the substrate and luciferase conversion of aminoluciferin. These simultaneous reactions generate a stable luminescent signal, which is proportional to caspase activity. Inclusion of the proteasome inhibitor MG-132 in the reagent eliminates nonspecific proteasome-mediated cleavage of the substrate, enabling sensitive measurement of caspase-1 activity.
