Bioneer ExiProgen His-tagged Protein Purification Kit (automated)
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
The 11-amino-acid HiBiT peptide tag can be added to a protein of interest using traditional cloning or CRISPR/Cas9 genome editing, enabling analysis of proteins under endogenous expression conditions. HiBiT-tagged proteins are typically measured using the bioluminescent signal generated by adding Nano-Glo HiBiT detection reagents, providing for precise measurement of changing protein levels over 7 logs of linear dynamic range. Adding the specific and highly sensitive Anti-HiBiT Monoclonal Antibody expands the detection options for HiBiT-tagged proteins, enabling traditional antibody-based methods for detecting the HiBiT tag and removing the need for tandem-tagging approaches.
