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Recombinant full-length human AMPK (combination of A2/B1/G1 subunits) was expressed by baculovirus in Sf9 insect cells using a C-terminal His tag. AMPK (A2/B1/G1) plays a key role in insulin signaling pathway and is a major therapeutic target for the treatment of diabetes (1). AMPK is viewed as a fuel sensor for glucose and lipid metabolism by modulating the activity of the autonomous nervous system in vivo. Short-term overexpression of a constitutively active form of AMPK in the liver leads to mild hypoglycemia and fatty liver due to increased fatty acid utilization (2). Kinase Enzyme System contains: Kinase: AMPK (A2/B1/G1), 10ug (Human, recombinant full-length). MW: ~69kDa (A2); ~38kDa (B1); ~40kDa (G1). Substrate: SAMStide (HMRSAMSGLHLVKRR); derived from the mouse acetyl-Coenzyme A carboxylase alpha (amino acids 73-85). Other: Reaction Buffer, DTT, AMP Solution. AMPK (A2/B1/G1) NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5563/.
