CyBio FeliX automated liquid handler
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The ADP-Glo Max Assay is a luminescent ADP detection assay that provides a universal, homogeneous, high-throughput screening method to measure ATPase or kinase activity by quantifying the amount of ADP produced in a reaction. The assay can be used to monitor the activity of virtually any ADP-generating enzyme (e.g., kinase or ATPase) when higher ATP concentration is required (up to 5mM). The two-step assay produces a strong signal that positively correlates with enzyme activity and can be adapted to a multitude of plate formats. The assay has a high dynamic range and produces a strong signal at low ATP-to-ADP conversion, making it well suited for screening low-activity ATPases such as drug membrane transporters and heat-shock proteins. The assay produces minimal false hits and Z' values of greater than 0.7.
