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Recombinant human VRK2 (1-375) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. VRK2 (also known as vaccinia related kinase 2) is a member of the vaccinia-related kinase (VRK) family of serine/threonine protein kinases. VRK2 is widely expressed in human tissues and highly expressed in actively dividing cells, such as those in testis, leukocytes, fetal liver and carcinomas (1). VRK2 can be used to phosphorylate casein and itself undergo autophosphorylation. VRK2 interacts specifically with Epstein-Barr virus BHRF1, a homologue of Bcl-2, and this interaction is involved in protecting cells from apoptosis (2). Kinase Enzyme System contains: Kinase: VRK2, 10ug (Human, recombinant; amino acids 1-375). MW: ~66kDa. Substrate: Native Casein Protein was purified from bovine milk. Other: Reaction Buffer, DTT, MnCl2. VRK2 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/7444/.
