Carousel pipette stand for up to 6 mechanical pipettes, except bravo
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Recombinant human SIK (1-303) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. SIK is a protein kinase that is involved in regulating AMPK-related kinases (1). SIK may mediate the physiological effects of LKB1, including its tumour suppressor function. SIK is also involved in signaling by various proteins like STRAD, NUAK1, NUAK2, BRSK1, BRSK2, QIK, QSK, SIK, MARK1, MARK2, MARK3, MARK4 and MELK that are related to AMPK. Activation of SIK1 by phosphorylation on Thr322 can lead to an increase in the catalytic activity of sodium/potassium ATPase alpha subunit at the plasma membrane (2). This results in an increase in intracellular sodium in intact mammalian cells. Kinase Enzyme System contains: Kinase: SIK, 10ug (Human, recombinant; amino acids 1-303). MW: ~36kDa. Substrate: AMARA peptide (AMARAASAAALARRR). Other: Reaction Buffer, DTT. SIK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/150094/.
