Pestle, 0.5ml, Assorted, 400/Case
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Full-length rhPKCbeta I was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. A member of the PKC family (phospholipid-dependent serine/threonine kinase), it is highly related to PKCbetaII. Unlike the mature PKCbetaII mRNA and protein, which rapidly increase following acute insulin treatment, PKCbetaI mRNA and protein levels remain unchanged. The stable overexpression of PKCbetaI, but not PKCbetaI, leads to insulin-stimulated glucose uptake into cells. Upon stimulation of B lymphocytes and mast cells, Syk regulates Btk, and Btk selectively regulates enzymatic activity of PKCbetaI. Specific regulation of PKCbetaI by Btk is consistent with the selective association of Btk with PKCbetaI. Kinase: PKCbetaI, 10ug (Human, recombinant full length). MW: ~102kDa. Substrate: PKCtide (ERMRPRKRQGSVRRRV); derived from protein kinase C epsilon (amino acid 149-164). Reaction Buffer: DTT, Lipid Activator Solution. PKC?I NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5579/.
