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Recombinant human MYO3 beta (1-326) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. MYO3 beta is a member of the Class III myosins (actin-dependent motor proteins containing an amino-terminal kinase domain) and contains an N-terminal kinase domain, followed by motor, neck and tail domains. The MYO3 beta gene generates a variety of splice variants that contain 1 or 2 calmodulin-binding (IQ) motifs in the neck domain and 1 of 3 domains in the tail domain. Northern blot analysis shows expression of a 7kb MYO3 beta transcript in the human retina but not in the RPE cell line (1). The MYO3 beta gene transcript is also detected in the kidney, intestine and testis. The MYO3 beta gene maps to chromosome 2q31.1-q31.2 by genomic sequence alignment. Contains: Kinase: MYO3 beta, 10ug (Human, recombinant; amino acids 1-326). MW: ~63kDa. Substrate: Native Swine Myelin Basic Protein. Reaction Buffer, DTT. MYO3 beta NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/140469/.
