CyBio Drop automated liquid handler
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Recombinant human MINK1 (1-320) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. MINK1 is a member of the germinal center family of kinases that are homologous to the Ste20 family and regulate a wide variety of cellular processes, including cell morphology, cytoskeletal rearrangement and survival. Overexpression of kinase-dead mutants of MINK1 leads to enhanced cell spreading, actin stress fiber formation, adhesion to extracellular matrix and decreased cell motility and invasion. MINK is activated after Ras induction via a mechanism involving reactive oxygen species and mediates stimulation of p38 MAPK downstream of the Raf/ERK pathway. Kinase Enzyme System contains: Kinase: MINK1, 10ug (Human, recombinant; amino acids 1-320). MW: ~61kDa. Substrate: Native Swine Myelin Basic Protein (MBP). Other: Reaction Buffer, DTT. MINK1 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/50488/.
