Carousel pipette stand for up to 6 mechanical pipettes, except bravo
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Full-length rhIKKbeta was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. IKKbeta is a serine/threonine protein kinase that phosphorylates the I-kappa-B protein, an inhibitor of the transcription factor NF-kappa-B complex. Phosphorylation of I-kappa-B triggers the degradation of the inhibitor via the ubiquitination pathway, thereby activating NF-kappa-B complex. IKKbeta activity is stimulated by TNF and IL1; IKKbeta forms a heterodimer that interacts with NIK. Overexpression of catalytically inactive IKKbeta blocks cytokine-induced NF-kappa-B activation. Aspirin and sodium salicylate can specifically inhibit IKKbeta activity in vitro and in vivo by binding to IKKbeta to reduce ATP binding. Contains: Kinase: IKKbeta, 10ug (Human, recombinant full-length). MW: ~105kDa. Substrate: IKKtide (KKKKERLLDDRHDSGLDSMKDEE); derived from human IkBA (amino acids 21-41). Other: Reaction Buffer, DTT. IKKbeta NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/3551/.
