Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human FGFR4 (460-end) was expressed by baculovirus in Sf9 cells using an N-terminal GST tag. FGFR4 is a member of the fibroblast growth factor receptor family, which play a role in mitogenesis and differentiation (1). FGFR4 preferentially binds acidic fibroblast growth factor and is overexpressed in gynecological tumor samples, suggesting a role in breast and ovarian tumorigenesis. FGFR4 gene expression is upregulated in doxorubicin-treated, apoptosis-resistant cancer cell clones (2). Ectopic expression of FGFR4 in cancer cells leads to reduced apoptosis sensitivity on treatment with doxorubicin or cyclophosphamide, whereas knockdown of endogenous FGFR4 expression in breast cancer cell lines has the opposite effect. Kinase Enzyme System contains: Kinase: FGFR4, 10ug (Human, recombinant; amino acids 460-end). MW: ~65kDa. Substrate: Poly (4:1 Glu, Tyr) peptide. Other: Reaction Buffer, DTT, MnCl2. FGFR4 NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/2264/.
