Bioneer AccuRapid Cell-Free Protein Expression Kit
Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human FAK (amino acids 393-698) was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. FAK (Focal Adhesion Kinase) is a nonreceptor protein tyrosine kinase involved in signal transduction from integrin-enriched focal adhesion sites that mediate cell contact with the extracellular matrix. FAK-enhanced signals have been shown to mediate the survival of anchorage-dependent cells and are critical for efficient cell migration in response to growth factor receptor and integrin stimulation. Elevated expression of FAK in human tumors has been correlated with increased malignancy and invasiveness. Elevated FAK expression in anaplastic astrocytoma and glioblastoma tumor biopsy samples has been demonstrated. Kinase Enzyme System contains: Kinase: FAK, 10ug (Human, recombinant; amino acids 393-698). MW: ~35kDa. Substrate: Poly (4:1 Glu, Tyr) Peptide. Other: Reaction Buffer, DTT, MnCl2. FAK NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/5747/.
