Freephone: 0800 257 428 Email: sales@alphatech.co.nz
Recombinant human EGFR (L858R) (amino acids 695-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EGFR is a transmembrane glycoprotein with tyrosine kinase activity. Binding of EGF to EGFR induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation, differentiation, motility and cell survival. Activation of EGFR triggers mitogenic signaling in gastrointestinal mucosa; its expression is upregulated in colon cancers and most neoplasms. Activation of EGFR triggers activation of ERK-signaling pathway in normal gastric epithelial and colon cancer cell lines. Inactivation of EGFR with selective inhibitors significantly reduces ERK2 activation, c-fos mRNA expression and cell proliferation. Kinase Enzyme System contains: EGFR (L858R), 10ug (Human, recombinant; amino acids 695-end). MW: ~89kDa. Substrate: Poly (4:1 Glu, Tyr) peptide. Reaction Buffer, MnCl2, DTT. EGFR (L858R) NCBI Database Entry: www.ncbi.nlm.nih.gov/gene/1956/.
